Effects of N-Hibit™ Seed Treatment on Soybean Yields – 2008 Iowa Research

December 8, 2008
ICM News

By Greg Tylka and Chris Marett, Department of Plant Pathology

Harpin protein is a natural plant compound that can stimulate plant defense responses. And N-Hibit  is a seed-treatment containing harpin protein that is sold in the United States for management of the soybean cyst nematode (SCN).  Iowa State University Extension evaluated the effects of N-Hibit  seed treatment on soybean yield and SCN population densities in nine field experiments located throughout Iowa in 2007 and in nine different field experiments in 2008. The work was supported by the soybean checkoff through funds from the Iowa Soybean Association.

In 2008, the experiments were conducted in Laurens, Mason City and Winthrop in northern Iowa; Gowrie, Nevada, and Urbana in central Iowa; and Council Bluffs, Hills, and Malvern in southern Iowa.

Figure 1. 2008 Experiment Locations

In both years, an SCN-susceptible and an SCN-resistant variety were grown at each experiment, and the seeds of each of the two varieties were either left untreated or were treated with N-Hibit  at a rate recommended by Plant Health Care Inc., the distributors of the product. All of the plots were four 17-foot-long rows spaced 30 inches apart. There were four replicate plots per variety-seed treatment combination, and 16 plots total per experiment. The center two rows of each four-row plot were harvested with a plot combine, total seed weight per plot and seed moisture were determined, and total plot seed weights were converted to bushels per acre.

Soil samples were collected from the plots to determine the SCN population densities. Ten soil cores were collected from the center two rows of each plot immediately after planting and again at the time of harvest. SCN cysts were extracted from a 100-cc subsample (a little less than a half cup) of each soil sample, and SCN eggs were extracted from the cysts and counted. 

The yield results from all experiments and the end-of-season SCN egg population densities from three of the nine locations are presented in the table below.

Table 1. 2008 Results - N-Hibit  seed treatment experiments in nine Iowa locations.

In 2008, N-Hibit™  did not significantly affect the yield of the SCN-resistant or the SCN-susceptible soybean variety at any of the nine experimental locations. But overall, yields of the SCN-resistant varieties were significantly greater than the SCN-susceptible varieties in the experiments at Council Bluffs, Malvern, Mason City, and Urbana.

At this time (early December 2008), the final (end-of-season) SCN population densities are available only for three of the experiments (Gowrie, Hills, and Winthrop) conducted in 2008.  In those experiments, there was no significant difference in final SCN egg population densities in plots of SCN-susceptible or SCN-resistant soybean varieties left untreated or treated with N-Hibit .  However, at all three locations, there were significantly greater final SCN population densities on the SCN-susceptible soybean varieties than the resistant varieties.

In the experiments conducted in 2007, N-Hibit  had no effect on yield of the SCN-resistant soybeans at any of the experiments and no effect on yield of the susceptible soybean varieties at seven of the nine locations. Yields of N-Hibit -treated susceptible soybean varieties were 2.1 and 3.0 bushels per acre greater than yields of untreated soybeans at the Melrose and Urbana experiments, respectively, in 2007.

When the end-of-season SCN egg population density data are obtained for all nine of the experiments conducted in 2008, a revised report of this work will be published in Integrated Crop Management News that will include all nematode data from 2008 and also a combined analysis of yield and SCN egg population density data from all 18 experiments conducted throughout Iowa in 2007 and 2008.


Greg Tylka is a professor of plant pathology with extension and research responsibilities in management of plant-parasitic nematodes. Chris Marett is an assistant scientist with responsibilities for research on the biology and management of the soybean cyst nematode.


Links to this article are strongly encouraged, and this article may be republished without further permission if published as written and if credit is given to the author, Integrated Crop Management News, and Iowa State University Extension and Outreach. If this article is to be used in any other manner, permission from the author is required. This article was originally published on December 8, 2008. The information contained within may not be the most current and accurate depending on when it is accessed.


Greg Tylka Morrill Professor

Dr. Greg Tylka is a Morrill Professor in the Department of Plant Pathology and Microbiology at Iowa State University with extension and research responsibilities for management of plant-parasitic nematodes. The focus of Dr. Tylka's research program at Iowa State University is primarily the soybea...